Shared posts

22 Aug 01:29

[ASAP] Feedback-Induced and Oscillating pH Regulation of a Binary Enzyme–Polymersomes System

by Xueyi Wang, Silvia Moreno, Susanne Boye, Ping Wen, Kehu Zhang, Petr Formanek, Albena Lederer, Brigitte Voit, and Dietmar Appelhans

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Chemistry of Materials
DOI: 10.1021/acs.chemmater.1c00897
26 Jul 08:06

[ASAP] Lipids and Lipid Derivatives for RNA Delivery

by Yuebao Zhang, Changzhen Sun, Chang Wang, Katarina E. Jankovic, and Yizhou Dong

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Chemical Reviews
DOI: 10.1021/acs.chemrev.1c00244
08 Apr 03:26

Artificial Organelles with Orthogonal‐Responsive Membranes for Protocell Systems: Probing the Intrinsic and Sequential Docking and Diffusion of Cargo into Two Coexisting Avidin–Polymersomes

by Xueyi Wang, Silvia Moreno, Susanne Boye, Peng Wang, Xiaoling Liu, Albena Lederer, Brigitte Voit, Dietmar Appelhans
Artificial Organelles with Orthogonal-Responsive Membranes for Protocell Systems: Probing the Intrinsic and Sequential Docking and Diffusion of Cargo into Two Coexisting Avidin–Polymersomes

For studying orthogonal-responsive membranes in eukaryotic protocells, artificial organelles are constructed by two different pH-stable and pH-responsive avidin–polymersome biohybrid structures. Different pH-dependent “open”, “closing/opening”, and “closed” membrane states of the two coexisting biohybrid structures pave the way to different intrinsic and sequential docking and diffusion processes of cargo (macro)molecules in a pH- and size-dependent manner.


Abstract

The challenge of effective integration and use of artificial organelles with orthogonal-responsive membranes and their communication in eukaryotic protocells is to understand the intrinsic membrane characteristics. Here, a novel photo-crosslinked and pH-responsive polymersome (Psome B) with 2-(N,N′-diisopropylamino)ethyl units in the membrane and its respective Avidin-Psome B hybrids, are reported as good candidates for artificial organelles. Biotinylated (macro)molecules are able to dock and diffuse into Avidin-Psome B to carry out biological activity in a pH- and size-dependent manner. Combined with another polymersome (Psome A) with 2-(N,N′-diethylamino)ethyl units in the membrane, two different pH-responsive polymersomes for mimicking different organelles in one protocell system are reported. The different intrinsic docking and diffusion processes of cargo (macro)molecules through the membranes of coexisting Psome A and B are pH-dependent as confirmed using pH titration–dynamic light scattering (DLS). Psome A and B show separated “open”, “closing/opening”, and “closed” states at various pH ranges with different membrane permeability. The results pave the way for the construction of multicompartmentalized protocells with controlled communications between different artificial organelles.

10 Apr 13:08

A Force to Be Reckoned With: A Review of Synthetic Microswimmers Powered by Ultrasound

by K. Jagajjanani Rao, Fei Li, Long Meng, Hairong Zheng, Feiyan Cai, Wei Wang

Synthetic microswimmers are a class of artificial nano- or microscale particle capable of converting external energy into motion. They are similar to natural microswimmers such as bacteria in behavior and are, therefore, of great interest to the study of active matter. Additionally, microswimmers show promise in applications ranging from bioanalytics and environmental monitoring to particle separation and drug delivery. However, since their sizes are on the nano-/microscale and their speeds are in the μm s−1 range, they fall into a low Reynolds number regime where viscosity dominates. Therefore, new propulsion schemes are needed for these microswimmers to be able to efficiently move. Furthermore, many of the hotly pursued applications call for innovations in the next phase of development of biocompatible microswimmers. In this review, the latest developments of microswimmers powered by ultrasound are presented. Ultrasound, especially at MHz frequencies, does little harm to biological samples and provides an advantageous and well-controlled means to efficiently power microswimmers. By critically reviewing the recent progress in this research field, an introduction of how ultrasound propels colloidal particles into autonomous motion is presented, as well as how this propulsion can be used to achieve preliminary but promising applications.

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Ultrasound-powered microswimmers are a type of self-powered nano- or microparticle that has attracted much attention recently. Due to their biocompatibility and versatile functionalities, these microswimmers are of great interest to a wide spectrum of readers. In this article, the recent advances in various aspects of ultrasound-powered microswimmers are critically reviewed, including their propulsion mechanisms, functionalities, and applications.

05 Oct 11:32

Tailored Freestanding Multilayered Membranes Based on Chitosan and Alginate

by Joana M. Silva, Ana Rita C. Duarte, Sofia G. Caridade, Catherine Picart, Rui L. Reis and João F. Mano

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Biomacromolecules
DOI: 10.1021/bm501156v