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31 Jan 02:35

Ep. 464 - McCabe And Mrs. Clinton

The deputy FBI director resigns – or was he fired? Republicans vote to Release The Memo, and Democrats lose it. And it’s State Of The Union time! Date: 01-30-2018
15 Sep 21:48

Hr1: Hurricane Irma, Cassini Farewell

by Science Friday

Biologists wait to assess the damage done to a delicate ecosystem by Irma. And in 13 years, the Cassini orbiter showed us lakes on Titan, geysers on Enceladus, and a new understanding of all things Saturn.

07 Jun 22:20

Needle adapters for intradermal administration of fractional dose of inactivated poliovirus vaccine: Evaluation of immunogenicity and programmatic feasibility in Pakistan

Publication date: 31 May 2017
Source:Vaccine, Volume 35, Issue 24
Author(s): Ali Faisal Saleem, Ondrej Mach, Mohammad T. Yousafzai, Asia Khan, William C. Weldon, M. Steven Oberste, Roland W. Sutter, Anita K.M. Zaidi
Administration of 1/5th dose of Inactivated poliovirus vaccine intradermally (fIPV) provides similar immune response as full-dose intramuscular IPV, however, fIPV administration with BCG needle and syringe (BCG NS) is technically difficult. We compared immune response after one fIPV dose administered with BCG NS to administration with intradermal devices, referred to as Device A and B; and assessed feasibility of conducting a door-to-door vaccination campaign with fIPV. In Phase I, 452 children 6–12months old from Karachi were randomized to receive one fIPV dose either with BCG NS, Device A or Device B in a health facility. Immune response was defined as seroconversion or fourfold rise in polio neutralizing antibody titer 28days after fIPV among children whose baseline titer ≤362. In Phase II, fIPV was administered during one-day door-to-door campaign to assess programmatic feasibility by evaluating vaccinators’ experience. For all three poliovirus (PV) serotypes, the immune response after BCG NS and Device A was similar, however it was lower with Device B (34/44 (77%), 31/45 (69%), 16/30 (53%) respectively for PV1; 53/78 (68%), 61/83 (74%), 42/80 (53%) for PV2; and; 58/76 (76%), 56/80 (70%), 43/77 (56%) for PV3; p<0.05 for all three serotypes). Vaccinators reported problems filling Device B in both Phases; no other operational challenges were reported during Phase II. Use of fIPV offers a dose-saving alternative to full-dose IPV.

10 Jun 00:39

240GB SanDisk Extreme Pro 2.5" SATA III Solid State Drive $90 + Free Shipping

by iconian
240GB SanDisk Extreme Pro 2.5" SATA III Solid State Drive  $90 + Free Shipping

Thumb Score: +30
Adorama has 240GB SanDisk Extreme Pro 2.5" SATA III Solid State Drive SSD (SDSSDXPS-240G-G25) for $103.99 - $14.04 w/ promo code STEXP0616 = $89.95. Shipping is free. Thanks iconian

Note, promo code is applied on the final checkout page.

Deal Editor's Notes & Price Research: This 7mm SSD features a 10-year warranty. -johnny_miller
23 Sep 23:26

Transcriptome analysis of Zymomonas mobilis ZM4 reveals mechanisms of tolerance and detoxification of phenolic aldehyde inhibitors from lignocellulose pretreatment

by Xia Yi
Background: Phenolic aldehydes generated from lignocellulose pretreatment exhibited severe toxic inhibitions on microbial growth and fermentation. Numerous tolerance studies against furfural, 5-hydroxymethyl-2-furaldehyde (HMF), acetate, and ethanol were reported, but studies on inhibition of phenolic aldehyde inhibitors are rare. For ethanologenic strains, Zymomonas mobilis ZM4 is high in ethanol productivity and genetic manipulation feasibility, but sensitive to phenolic aldehyde inhibitors. Molecular mechanisms of tolerance for Z. mobilis toward phenolic aldehydes are not known. Results: We took the first insight into genomic response of Z. mobilis ZM4 to the phenolic aldehyde inhibitors derived from lignocellulose pretreatment. The results suggest that the toxicity to cells is caused by the functional group of phenolic aldehyde, similar to furfural and HMF, rather than aromatic groups or phenolic hydroxyl groups. Transcriptome response against 4-hydroxybenzaldehyde, syringaldehyde, and vanillin, representing phenolic groups H, S, and G, respectively, was investigated. The atlas of the important genes responsible for significantly enhanced and repressed genes at the genomic level was illustrated. 272 genes with twofold greater expressions than non-treated controls and 36 gene clusters in response to challenges of these phenolic aldehydes were identified. Several reductases encoded by ZMO1116, ZMO1696, and ZMO1885 were found to play the key roles in reducing phenolic aldehydes into the corresponding phenolic alcohols. Reduction of phenolic aldehydes by overexpression of ZMO1116, ZMO1696, and ZMO1885 in Z. mobilis ZM4 resulted in the increased inhibitor conversion and ethanol productivity, especially for 4-hydroxybenzaldehyde and vanillin. Several transporter genes such as ZMO0282, ZMO0283, ZMO0798, ZMO0799, and ZMO0800 was also displayed significantly increased expressions against the phenolic aldehydes. Conclusions: The genes encoding reductases are with potentials on phenolic aldehydes-tolerant genes contributing to the reduction of phenolic aldehydes into the corresponding phenolic alcohols forms for Z. mobilis ZM4. Overexpression of the key genes improved the conversion ratio and ethanol productivity of 4-hydroxybenzaldehyde and vanillin with high toxicity. New knowledge obtained from this research aids understanding the mechanisms of bacterial tolerance and the development of the next-generation biocatalysts for advanced biofuels production.
31 Aug 20:59

Microbe produces ethanol from switchgrass without pretreatment

Scientists engineered a strain of a consolidated bioprocessing bacterium that breaks down biomass without pretreatment, producing ethanol and demonstrating the successful conversion of switchgrass cellulosic biomass.